. 2024 Sep 16;20(9):e1012560. doi: 10.1371/journal.ppat.1012560

Table 1. Kinetic parameters of the fitted models to the enzymatic activity data of ChiA or ChiB towards the pseudo-chitin substrates 4-MU-GlcNAc₂, 4-MU-GlcNAc₃ or colloidal chitin.

K_cat = catalytic constant, K_m = substrate concentration given that the reaction rate reaches ½ V_max, K_cat/K_m = Specificity constant, ND: kinetic parameters not determined due to unambiguous model fitting for ChiB using 4-MU-(GlcNAc)₃. BDL: “Below detection limit”.

	ChiA			ChiB
	K_cat (s^-1)	K_m (μM)	K_cat/K_m (s^-1 μM^-1)	K_cat (s^-1)	K_m (μM)	K_cat/K_m (s^-1 μM^-1)
GlcNAc	BDL	BDL	BDL	BDL	BDL	BDL
GlcNAc ₂	3.09	145.8 ± 91.3	0.021	15.2	24.3 ± 9.7*10⁻³	0.627
GlcNAc ₃	0.0993	2.6 ± 2.3*10⁻⁴	0.039	ND	ND	ND
Chitin	0.176	14.8 ± 6.2	0.012	0.263	6.6 ± 1.2	0.040

Table 1. Kinetic parameters of the fitted models to the enzymatic activity data of ChiA or ChiB towards the pseudo-chitin substrates 4-MU-GlcNAc2, 4-MU-GlcNAc3 or colloidal chitin.

Table 1. Kinetic parameters of the fitted models to the enzymatic activity data of ChiA or ChiB towards the pseudo-chitin substrates 4-MU-GlcNAc₂, 4-MU-GlcNAc₃ or colloidal chitin.