Figure 5. Liver and muscle mTORC1 activity is not constitutively repressed by CR.

(A-B) Experimental design. Night-fed male mice were placed in new cages without food at ZT15 (A) and Morning-Fed mice at ZT3 (B). (C-D) Western analysis of Liver Night-Fed (C) and Morning-Fed (D)male mice. (E-F) Western analysis of Muscle Night-Fed (E) and Morning-Fed (F) male mice. (C-F) n = 6 mice per diet per time point; *p<0.05, Sidak’s test post 2-way ANOVA). Data represented as mean ± SEM. See also Supplementary Figures 11–13.