Fig. 3.
(A) Phylogenetic tree based on 16S rRNA gene sequences of the genus Nitrobacter. Rhodopseudomonas pseudopalustris DSM123T was inserted as an outgroup. The tree was constructed using the maximum-likelihood method. Bootstrap values at the branch nodes were iterated 1,000 times. The strain obtained in the present study is shown in bold. The scale bar corresponds to 0.5% estimated sequence divergence. Accession numbers are shown to the right of the organism names in brackets. Cluster numbers from 1 to 5 are shown based on a previous study (Vanparys et al., 2007). (B) Phylogenetic tree based on the nxrB1 gene sequences of the genus Nitrobacter. A Nitrococcus mobilis Nb-231T nxrB homologue was used as an outgroup. The tree was constructed using the maximum-likelihood method. Bootstrap values at the branch nodes were iterated 1,000 times. The strain obtained in the present study is shown in bold. The scale bar corresponds to 5% estimated sequence divergence. Accession numbers are shown to the right of the organism names in brackets. Cluster numbers from 1 to 5 are shown based on a previous study (Vanparys et al., 2007).