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. 2024 Sep 20;14(18):e5074. doi: 10.21769/BioProtoc.5074

Figure 2. Steps of isolating anterior limbal stroma.

Figure 2.

(A) Whole adult cornea (~12 mm diameter) without the central region after removal by a circular surgical trephine. (B–C) Cut the corneal rim into quadrants. (D–F) Trimming of limbal strip with ~0.5 mm on scleral side and 1–1.5 mm on corneal side. (G–I) Making vertical cuts to separate anterior limbal stroma from the rest of tissue. (J) Separation of anterior limbal stroma. (K) Cuts of anterior limbal stromal strip into small blocks for efficient collagenase digestion. *denotes the anterior limbal stroma.