| Reagent | Final concentration | Quantity |
|---|---|---|
| Recombinant human EGF | 10 mg/mL | 100 mL from 0.5 mg/mL stock |
| DMEM/F12 | 1× | 5 mL |
| Flexbumin | 0.1% (v/v) | 20 mL from 25% stock |
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