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. 2024 May 8;20(10):1341–1352. doi: 10.1038/s41589-024-01612-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, HONE1 cells with or without fibrin gel were implanted subcutaneously into female BALB/c nude mice to construct xenograft growth models and were exposed to radiotherapy (8 Gy, arrow). Tumor volumes for each group are shown (n = 5). b, Hematoxylin and eosin (H&E) staining and IHC of fibrin, PCK2 and ACSL4 pT679 showing the presence of fibrin gel and the level of PCK2 and ACSL4 pT679 in TRC tumors and bulk tumors treated with radiotherapy. Images are representative of n = 5 images. Scale bars, 100 µm. c, Flow cytometric analysis of the proportion of EpCAM⁺pSTAT3⁺ in TRC tumors and bulk tumors treated with radiotherapy. n = 5. d, Representative immunofluorescence images of PCK2 and ACSL4 pT679 in EpCAM⁺integrin β3⁺ and EpCAM⁺integrin β3⁻ cells. Scale bars, 10 µm. Images are representative of n = 10 images. e, The content of esterified AA (C20:4)-PE molecular species in integrin β3⁺ and integrin β3⁻ tumor cells isolated from radiation-treated HONE1 tumors. n = 5. f,g, The content of PE (38:4)-OOH (f) and PE (40:4)-OOH (g) in integrin β3⁺ and integrin β3⁻ tumor cells isolated from radiation-treated HONE1 tumors. n = 5. h, HONE1 cells stably transfected with indicated plasmids were implanted subcutaneously into female BALB/c nude mice to construct xenograft growth models. Indicated groups were exposed to radiotherapy (8 Gy, arrow). All groups were provided with a Dox drink (100 mg kg⁻¹, intragastrical administration, every 2 d, starting from ‘arrowhead’ until the endpoint). Tumor volumes for each group are shown (n = 5). i, PCK2^Tet HONE1 cells with or without fibrin gel were implanted subcutaneously into female BALB/c nude mice to construct xenograft growth models. Indicated groups were exposed to radiotherapy (8 Gy, arrow). Mice were provided with a Dox drink (100 mg kg⁻¹, intragastrical administration, every 2 d, starting from ‘arrowhead’ until the endpoint) or a normal drink. Tumor volumes for each group are shown (n = 5). Data are shown as mean ± s.e.m. (a,h,i) or mean ± s.d. (c,e–g); paired two-tailed t-test (e), unpaired two-tailed t-test (c,f,g) or two-way ANOVA (a,h,i).

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