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. 2024 May 8;20(10):1341–1352. doi: 10.1038/s41589-024-01612-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Immunoblots showing the expression of GPX4 and SLC7A11 in TRCs and bulk cells. b, GSH/GSSG ratios in TRCs and bulk cells. c, The relative mRNA expression of AIFM2 (encoding FSP1 protein) in TRCs and bulk tumor cells. d, NADH consumption assay with CoQ₁ to determine the FSP1 activity. e, Representative results of a cellular thermal shift assay (CETSA). FSP1 protein was resistant to heat denaturation in the presence of CoQ₁₀ (upper) and α-tocopherol (lower). f, Representative results of a cellular thermal shift assay (CETSA). FSP1 protein from HONE1 bulk tumor cells and TRCs showed similar heat denaturation in the presence of CoQ₁₀ (upper) and α- tocopherol (lower). g-h, The relative mRNA expression of ALOX15 (i) or PEBP1(j) in TRCs and bulk tumor cells. P = 0.1556, 0.0683, 0.4532, 0.1007, 0.1243 and 0.7519. i, The cellular ferrous iron content of TRCs and bulk tumor cells was measured. P = 0.5932, 0.3396 and 0.6071. j, The relative mRNA expression of ACSL1, ACSL3, ACSL4, FATP2, LPCAT3, PLA2G6, PLA2G2F and PLA2G4A in TRCs and bulk tumor cells from HONE1 cells. Data are shown as mean ± SD, unpaired two-tailed t-test (b-c, g- j), n = 3 independent experiments. Bulk, black points; TRC, red points. One of three experiments is shown (a, e-f). ns, not significant.

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