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. 2024 Aug 31;8(10):ziae113. doi: 10.1093/jbmrpl/ziae113

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© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society for Bone and Mineral Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Study design. Overview of experimental design. Four-month-old female Sprague–Dawley rats underwent a sham operation or ovariectomy (OVX) surgery to induce estrogen-deficiency. Animals were aged for 2 mo to allow for bone depletion. At 6 mo of age, animals underwent: (1) unilateral extraction of the maxillary first molar (M1) and sacrifice after 42 d socket healing; or (2) bilateral extraction of the maxillary M1 and received press-fit dental implants placed in osseous well-shaped defects at 42 d, followed by biopsy harvest at 14- or 21-d post-implant placement.