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. 2024 Sep 23;7(3):pbae018. doi: 10.1093/pcmedi/pbae018

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© The Author(s) 2024. Published by Oxford University Press on behalf of the West China School of Medicine & West China Hospital of Sichuan University.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — High metastatic NPC cell-derived exosomes confer migration and invasion ability on low metastatic cells. Images (A) and statistical diagrams (B) showing the migration and invasion ability of CNE-2 cells treated with CM from different subtypes of cells. (C) Western blot identification of exosome markers including ALIX, CD9, and CD63; the cell marker GM130 was used as a negative control. (D) Diagram showing the distribution of diameter of the exosomes detected by Nanosight NS300. (E) TEM images detecting phosphotungstic acid-stained exosomes. (F) Fluorescent images of the CNE-2 cells treated with exosomes stained with PKH67. Images (G) and statistical diagrams (H) showing the migration and invasion ability of CNE-2 cells treated with the indicated components. ns, p>0.05; ***, p<0.001.