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. 2024 Sep 5;13(9):846. doi: 10.3390/antibiotics13090846

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The comparative BGC of NNS5-6 AMP was proposed by the non-ribosomal peptide synthesis in antiSMASH. The BGC was composed of core biosynthetic genes, regulatory genes, and transport-related genes. The translated protein from the core biosynthetic genes showed a similar amino acid sequence to fusaricidin synthetase in the DELTA-BLAST search within the NCBI database (red and grey colors indicate different and identical amino acids, respectively). The different amino acids in the gene product comparison are indicated by red color, as visualized by the NCBI MSA viewer (a). The similar amino acid sequences between NNS5-6 AMP and fusaricidin A–D were compared using multiple sequence alignment with MEGA X software (version 10.1.8) (b).