Table 1.
The lytic spectrum of PEF1 and relative lytic activities of endolysins LysPEF1-1 and LysPEF1-2 on 69 Enterococcus spp.
| Species | Strain Number | Phage PEF1 | Lytic Activity (%) | |
|---|---|---|---|---|
| LysPEF1-1 | LysPEF1-2 | |||
|
Enterococcus
faecalis |
J1 | ± | 66.32 ± 0.93 | 24.90 ± 4.06 |
| J2 | ± | 49.21 ± 19.91 | 5.80 ± 3.51 | |
| J3 | ± | 69.96 ± 2.62 | 6.85 ± 2.79 | |
| J4 | ± | 39.80 ± 0.60 | - | |
| J5 | − | - | - | |
| J6 | ± | 32.97 ± 6.70 | - | |
| J7 | ± | 56.83 ± 0.50 | 9.75 ± 1.16 | |
| J8 | + | 78.46 ± 1.54 | 31.36 ± 1.36 | |
| J9 | − | 62.44 ± 0.66 | 5.16 ± 0.96 | |
| J10 | ± | 73.74 ± 0.47 | 6.68 ± 0.02 | |
| J11 | + | 70.10 ± 2.73 | 10.95 ± 0.64 | |
| J12 | ± | 13.84 ± 1.34 | 5.00 ± 2.71 | |
| J13 | ± | 53.28 ± 0.93 | - | |
| J14 | − | - | - | |
| J15 | − | 53.34 ± 0.76 | - | |
| J16 | ± | 46.97 ± 18.04 | 8.04 ± 5.13 | |
| J17 | − | 67.62 ±5.55 | 1.41 ± 0.08 | |
| J18 | ± | 53.24 ± 0.32 | 8.15 ± 0.41 | |
| J19 | ± | 58.48 ± 14.42 | 8.58 ± 0.39 | |
| J20 | ± | 66.04 ± 12.23 | 8.85 ± 1.99 | |
| J21 | ± | 63.40 ± 7.80 | 20.13 ± 0.96 | |
| J27 | ± | 67.55 ± 1.81 | 38.62 ± 2.71 | |
| J28 | + | 58.27 ± 15.35 | 70.66 ± 1.99 | |
| J30 | ± | 45.27 ± 2.70 | 60.67 ± 5.74 | |
| J31 | ± | 73.28 ± 0.42 | 33.75 ± 3.58 | |
| J32 | ± | 61.49 ± 2.14 | 12.89 ± 2.13 | |
| J33 | ± | 54.4 ± 0.86 | 20.69 ± 0.07 | |
| J34 | ± | 35.31 ± 0.37 | 24.65 ± 0.21 | |
| JM9 | ± | 62.00 ± 2.03 | 30.59 ± 0.89 | |
| E1 | ± | 60.87 ± 1.65 | 13.80 ± 1.76 | |
| E2 | ± | 38.98 ± 3.97 | - | |
| E3 | ± | 20.22 ± 2.00 | - | |
| E4 | ± | 68.55 ± 5.63 | 69.10 ± 2.12 | |
| E5 | − | 57.65 ± 5.43 | 10.02 ± 0.76 | |
| E6 | ± | 46.52 ± 0.78 | 32.51 ± 5.01 | |
| E7 | ± | 61.84 ± 0.53 | 5.19 ± 0.91 | |
| E8 | ± | 64.48 ± 1.11 | 24.89 ± 0.50 | |
| E9 | ± | 10.41 ± 0.87 | 8.09 ± 1.78 | |
| E10 | ± | 78.70 ± 2.77 | 3.80 ± 0.19 | |
| E11 | ± | 25.21 ± 3.00 | 11.97 ± 0.56 | |
| E12 | ± | 73.37 ± 2.71 | 7.29 ± 1.29 | |
| E13 | ± | 74.89 ± 1.94 | 3.16 ± 1.78 | |
| E14 | + | 75.12 ± 6.12 | 29.36 ± 0.93 | |
| E15 | ± | 59.98 ± 2.87 | 3.52 ± 0.58 | |
| E16 | ± | 15.80 ± 5.12 | 1.40 ± 0.76 | |
| E17 | ± | 80.98 ± 1.98 | 1.31 ± 0.11 | |
| E18 | ± | 69.40 ± 6.20 | 2.10 ± 0.94 | |
| E19 | ± | 58.77 ± 3.02 | 4.60 ± 0.32 | |
| E20 | ± | 70.49 ± 1.99 | 8.55 ± 1.85 | |
| E21 | ± | 73.08 ± 2.10 | 6.55 ± 2.63 | |
| E22 | ± | 77.95 ± 4.10 | 40.93 ± 5.12 | |
| E23 | ± | 69.36 ± 2.08 | 6.55 ± 1.81 | |
| E24 | ± | 41.74 ± 5.34 | - | |
| E25 | ± | 80.07 ± 3.58 | 20.09 ± 1.96 | |
| E26 | ± | 81.23 ± 2.13 | 28.73 ± 0.47 | |
| E27 | ± | 80.42 ± 1.36 | 23.29 ± 2.14 | |
| E28 | ± | 51.98 ± 3.10 | 19.40 ± 0.86 | |
| E29 | ± | 77.46 ± 5.85 | 15.36 ± 0.68 | |
| E30 | ± | 12.34 ± 0.88 | 2.99 ± 0.76 | |
| JCM 7783T | ± | 65.34 ± 1.68 | 15.14 ± 1.29 | |
| JCM 5803T | ± | 8.56 ± 1.70 | 11.18 ± 29.30 | |
|
Enterococcus
faecium |
J22 | − | - | 14.47 ± 0.08 |
| J23 | ± | - | 29.58 ± 0.45 | |
| J24 | ± | - | 20.83 ± 0.39 | |
| J25 | − | 10.78 ± 0.82 | 5.16 ± 1.89 | |
| J26 | − | 3.25 ± 0.87 | 12.22 ± 0.73 | |
| J29 | − | - | 1.29 ± 0.71 | |
| J35 | − | - | - | |
| J36 | ± | 3.71 ± 1.02 | - | |
(+): Strong lysis, (±): Weak lysis, (−): Non-lysis or Lytic activity (%) < 0.