Table 2.
The lytic spectrum of PEF1 and relative lytic activities of endolysins LysPEF1-1 and LysPEF1-2 on six gram-positive and nine gram-negative strains.
| Species | Strain Number | Phage PEF1 | Lytic Activity (%) | |
|---|---|---|---|---|
| LysPEF1-1 | LysPEF1-2 | |||
| Staphylococcus aureus | NCTC 8325 | − | - | 2.20 ± 0.83 |
| No. 179 | − | - | 3.78 ± 1.64 | |
| Listeria monocytogenes | No. 185 | − | 23.59 ± 4.46 | 15.39 ± 1.39 |
| Bacillus cereus | BC-RI15 | − | - | 2.85 ± 0.82 |
| Clostridium perfrigens | JCM 1290T | − | 16.25 ± 1.44 | 9.72 ± 1.79 |
| S1 | − | 16.77 ± 0.57 | 10.02 ± 1.14 | |
| Escherichia coli | O157:H7 | − | - | - |
| Salmonella Typhimurium | IFO 12529 | − | 25.76 ± 7.42 | 26.35 ± 1.86 |
| S. Enteritidis | IFO 3313 | − | 12.25 ± 0.99 | 10.84 ± 0.42 |
| Campylobacter jejuni | L26 | − | 100.01 ± 0.22 | 101.51 ± 0.51 |
| C. coli | Can 10 | − | 87.29 ± 0.17 | 103.91 ± 0.59 |
| Pseudomonas alcaligenes | NBRC 14159 | − | 20.6 ± 1.00 | 19.24 ± 0.94 |
| P. fluorescens | NBRC 14160 | − | 13.18 ± 2.70 | 19.34 ± 6.41 |
| P. fragi | NBRC 3458 | − | - | 14.28 ± 0.63 |
| P. oleovorans | NBRC 13583 | − | 9.74 ± 1.26 | 21.37 ± 0.80 |
(−): Non-lysis or Lytic activity (%) < 0.