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. 2024 Sep 12;14(18):2651. doi: 10.3390/ani14182651

Table 1.

PCR primers and parameters used in this study.

Species Gene Primer ID Primer Sequences (5′-3′) Annealing Temperatures (°C) Fragment Length (bp)
T. buttreyi SSU rRNA FF GCGCCTGAGAGATAGCGACTA 59
RR GGACCTGTTATTGCTACCCTCTTC
bF GTTTTTTCTCAGGCAGCAATG 61 623
bR GCAACCTAGAAACCTAGGCG
P. hominis SSU rRNA F1 ATGGCGAGTGGTGGAATA 60
R1 CCCAACTACGCTAAGGATT
F2 TGTAAACGATGCCGACAGAG 60 339
R2 CAACACTGAAGCCAATGCGAGC