Skip to main content
. 2001 Jun;75(12):5711–5718. doi: 10.1128/JVI.75.12.5711-5718.2001

FIG. 2.

FIG. 2

Expression of LMP2A, Lyn, and Syk in LMP2A PY1PY2 and Y112F LCLs. (A) The protein levels of LMP2A, Lyn, Syk, and tubulin in BJAB cells (lanes 1) and in LMP2A− (lanes 2), LMP2A+ (lanes 3 and 4), and PY1PY2 (lanes 5 and 6) LCLs were analyzed. (B) Levels of Lyn and tubulin expression in LMP2A+ (lanes 1), PY1PY2 (lanes 2), and LMP2A Y112F (lanes 3 to 5) LCLs were analyzed. Triton X-100 lysates from the various cell lines were separated on sodium dodecyl sulfate–7.5% polyacrylamide gel electrophoresis gels, transferred to Immobilon membranes, and immunoblotted with anti-LMP2A, anti-Lyn, anti-Syk, or anti-tubulin antibody, followed by incubation with horseradish peroxidase-conjugated secondary antibody and enhanced chemiluminescence detection. Tubulin served as a protein loading control. The positions of LMP2A, Lyn (both the 56- and 53-kDa forms), Syk, and tubulin are indicated by arrows.