FIG. 5.
Induction of BZLF1 expression in LMP2A PY motif mutant LCLs. LCLs (4 × 106 cells) were untreated (−) or treated (+) with anti-human surface Ig (sIg) antibody for 48 h. Triton X-100 lysates from LCLs were separated on sodium dodecyl sulfate–7.5% polyacrylamide gel electrophoresis gels and immunoblotted with anti-BZLF1 antibody, followed by incubation with horseradish peroxidase-conjugated secondary antibody and enhanced chemiluminescence detection. Molecular mass standards are indicated (in kilodaltons).