FIG. 6.

Expression of LMP1 in LMP2A PY motif and tyrosine motif mutant LCLs. The protein levels of LMP1 was analyzed in various LMP2A phenylalanine-to-tyrosine-mutation LCLs. Triton X-100 lysates from PY1PY2 (lanes 1 and 2), Y23F (lanes 3 and 4), Y23F/Y31F (lane 5), Y31F (lane 6), Y60F (lanes 7 and 8), Y64F (lanes 9 and 10), Y74F (lanes 11 and 12), Y85F (lanes 13 and 14), Y101F (lanes 15 and 16), and Y112F (lanes 17 and 18) LCLs were separated on sodium dodecyl sulfate–7.5% polyacrylamide gel electrophoresis gels and immunoblotted with anti-LMP1 antibody. The position of LMP1 is indicated by an arrow, and molecular mass standards are indicated (in kilodaltons).