TABLE 1.
Serum-dependent cell growth of LMP2A PY mutant LCLsa
| Cell line | Growthb of cells in various serum concn (%)
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 × 105 cells
|
2.5 × 104 cells
|
5 × 103 cells
|
||||||||||
| 10 | 1 | 0.1 | 0 | 10 | 1 | 0.1 | 0 | 10 | 1 | 0.1 | 0 | |
| LMP2A− ES1 | +++ | +++ | ++ | − | +++ | ++ | − | − | +++ | − | − | − |
| LMP2A+ 65 | +++ | +++ | ++ | − | +++ | ++ | − | − | +++ | − | − | − |
| LMP2A+ 5 | +++ | +++ | + | + | +++ | + | − | − | +++ | − | − | − |
| PY1PY2 9.16 | +++ | ++ | + | − | +++ | + | − | − | +++ | − | − | − |
| PY1PY2 34 | +++ | +++ | ++ | ++ | +++ | +++ | − | − | +++ | − | − | − |
| PY1PY2 5 | +++ | +++ | ++ | + | +++ | + | − | − | +++ | − | − | − |
| PY1PY2 7 | +++ | +++ | +++ | ++ | +++ | ++ | − | − | +++ | − | − | − |
a
Cells were plated in 96-well tissue culture plates at either 105, 2.5 × 104, or 5 × 103 cells per well in 10, 1, 0.1, or 0% serum. The wells were scored 10 days after plating.
b
Growth was scored according to the following guidlines: +++, large clumps, acidic media, clear growth; ++, medium clumps, possible acidification of media, probable growth; +, small clumps, neutral or basic media, little growth; −, no clumps, neutral or basic media, no growth.