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. 2024 Sep 27;55:120. doi: 10.1186/s13567-024-01376-w

Figure 2.

Figure 2

P1/7 triggers macrophage pyroptosis and IL-1β secretion via NLRP3 and GSDMD. PECs from WT (C57BL/6) or Gsdmd−/− (C57BL/6) mice were uninfected or infected with P1/7 (MOI = 5) for 24 h. Western blotting was used to detect the expression of GSDMD and caspase-1 (A). ELISA was used to determine IL-1β secretion (B). PI uptake by PECs from WT and Gsdmd−/− mice was detected (C). In addition, PECs were pretreated with MCC950 (10 μM) for 1 h prior to infection. After infection, western blotting was used to detect the protein levels of caspase-1, GSDMD and IL-1β (D), and ELISA was used to determine the secretion of IL-1β (E). Furthermore, PECs were pretreated with Z-VAD-FMK (5 μM) for 1 h prior to infection. After infection, the protein levels of caspase-1, GSDMD and IL-1β (F) and the secretion of IL − 1β were determined (G). Moreover, images of PI uptake are shown (H). The data are presented as the mean ± SEM of three independent experiments with triplicate samples per experiment. *p < 0.05, **p < 0.01.