Figure 5.

DNMTs and SIRTs enzymatic activity after exposure to different concentrations of TMA. DNMTs activity (A) and SIRTs’ activity (B) were tested in a cell-free experimental setting after exposure of nuclear extract to different concentrations of TMA using the EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit (EpigenTek, Farmingdale, NY, USA) and the Universal SIRT Activity Assay Kit (Abcam, Waltham, MA, USA), respectively. TMA did not provoke any notable changes in DNMT activity at any of the concentrations tested (A). In contrast, the results indicate that TMA can hamper SIRTs’ activity in a concentration-dependent manner; the inhibition of their activity was significant within the concentration range of 0.1 µM to 1 mM (B). * p < 0.05; ** p < 0.01 vs. Control.