FIG. 1.

Molecular tools used for analyzing PV genomes. (A) Structure of the genome, showing the two noncoding regions (5′NC and 3′NC) covalently linked to the small viral protein VPg (3B) and to the poly(A) sequence (AAA), respectively. Genomic regions encoding viral proteins (VP4 to 3D) are indicated. Positions of the sequences used for strain-specific RT-PCR are shown as hatched boxes, and the corresponding pairs of primers are given. Names of RFLP assays and the corresponding genomic regions are indicated in grey boxes; the two assays used systematically are in bold. (B) Details of RFLP assays and genomic regions. Names of the primers used for RT-PCR are given (UG and UC denote genomic sense and complementary sense primers). Primer sequences are given in Materials and Methods. The nucleotide positions for amplicons are indicated according to Sabin 1 genome numbering.