TABLE 2.
Localization of recombination junctions by nucleotidic sequencing
| Type of junction | No. of junctions | Localizationa (nt) | Length (nt) | Nucleotide sequence (5′-3′) |
|---|---|---|---|---|
| S1/S3 | 1 | 5984–6009 | 26 | CAAGGTGAAATCCAGTGGATGAGACC |
| S2/S1 | 1 | 5036–5053 | 18 | CAGATCACTACAATGATT |
| S2/S1 | 1 | 5465–5493 | 29 | ATGGCTAAAAGAAACATTGTTACAGCAAC |
| S2/S1 | 1 | 6338–6363 | 26 | TACCCTTATGTAGCAATGGGAAAGAA |
| S2/S1 | 2 | 6389–6390 | 2 | AC |
| S2/S1 | 1 | 6464–6471 | 8 | GATGAACT |
| S2/S12nd | 1 | 6659–6672 | 14 | ATGGAAGAGAAGCT |
| S2/S1 | 2 | 6659–6672 | 14 | ATGGAAGAGAAGCT |
| S2/S1 | 1 | 6674–6678 | 5 | TTTGC |
| S2/S12nd | 1 | 6737–6744 | 8 | ATGGTGCT |
| S2/S12nd | 1 | 6854–6864 | 11 | TGCTCAGGCAC |
| S2/S1 | 1 | 6866–6894 | 29 | TACCCTTATGTAGCAATGGGAAAGAA |
| S2/S1 | 1 | 6953–6996 | 44 | ATGATTGCCTATGGTGATGATGTAATTGCTTCCTACCCCCATGA |
| S2/S32nd | 1 | 6530–6534 | 5 | AATGA |
| S2/S32nd | 1 | 6599–6608 | 8 | GTCACTGG |
| S2/S32nd | 1 | 6836–6858 | 23 | GGCGGCATGCCATCTGGCTGCTC |
| S2/S3 | 1 | 6860–6864 | 5 | GGCAC |
| S3/S1 | 1 | 4865–4869 | 5 | ATTCA |
| S3/S1 | 1 | 4904–4911 | 8 | AACATGGC |
| S3/S1 | 1 | 4927–4929 | 3 | GTG |
| S3/S1 | 1 | 5060–5076 | 17 | GAGAGAAACAGAAGATC |
| S3/S21st | 2 | 3404–3417 | 14 | AAAGCTGTGTACAC |
| S3/S21st | 1 | 3483–3492 | 10 | TCATGTGGAA |
| S3/S2 | 2 | 4514–4518 | 5 | CCAGG |
| S3/S2 | 2 | 4520–4536 | 17 | ACAGGAAAATCAGTTGC |
| S3/S21st | 2 | 4520–4536 | 17 | ACAGGAAAATCAGTTGC |
| S3/S21st | 1 | 4541–4542 | 2 | AA |
| S3/S21st | 1 | 4565–4584 | 20 | GAGAAAGAGAACACCTCCAC |
| S3/S2 | 1 | 4724–4728 | 5 | CCACC |
| S3/S2 | 5 | 4775–4800 | 26 | GTTTTAGCCTCCACCAACTCCAGTCG |
| S3/S2 | 2 | 4868–4872 | 5 | CAAGT |
| S3/S2 | 2 | 4901–4923 | 23 | CTCAACATGGCAATGGCTACTGA |
a
Recombination sites were localized (sequencing) in homologous segments flanked by two nucleotides that differentiate the genomes of the parental Sabin strains. Nucleotide (nt) numbering refers to Sabin 1 strain sequence.