Figure 5.

Prevalence of PGC-1a at the junction of old and new bone formation following cold therapy treatment. (A,E) at 2.5× magnification. Scale bar represents 500 µm. (B–D,F–H) at 20× magnification. Scale bar represents 50 µm. PGC-1a has been shown to be upregulated (I) at the junction of new and old bone. (F–H) Furthermore, PGC-1a expression within these junctions appears to be elevated following cold exposure (B–D) indicating PGC-1a upregulation. (I) Detected location of PGC-1α staining expression analysis (n = 8) of PGC-1α within cells at the junction of newly formed bone and old bone was 70.56% ± 7.08, while in non-junctional regions it was 29.44% ± 7.08 (* p-value < 0.00001).