Figure 8.

IVL DCM fraction inhibited the migration of A549 cells. (A) a confluent monolayer of A549 cells was wounded by scratching. The cells were then incubated with the indicated concentrations of IVL DCM fraction. The wound was imaged 24 and 48 h after treatment, and the images were analyzed to quantify cell migration using Image J software. Values represent the fold change in migration compared to the vehicle-untreated cells. Bar graphs in the lower panel in (A) represent the migration rate of the cells after 24 and 48 h of treatment. (B) Western blotting using anti-pFAK and anti-FAK antibodies. Values represent the average of three independent experiments (n = 3) and are expressed as mean ± SEM. ** p < 0.01, *** p < 0.001, and **** p < 0.0001.