Figure 5.

AQP1 immunostaining in the liver. AQP1 immunostaining was performed in the livers of wild-type mice with and without heat exposure and in Tie2-Cre/LNL-AQP1 dTG mice. AQP1-immunoreactions (irs) were ubiquitously observed in the hepatic vasculature, including the sinusoids of non-heat-exposed wild-type mice (Nor). It showed no staining in the portal triad’s (inset) hepatocytes and small bile ducts (asterisk). Minimal or no staining was observed in the primary antibody-free negative control (1st AB-free NC) in the livers of wild-type mice (Nor). There were no remarkable differences in AQP1-ir staining between wild-type (HS) and Tie2-Cre/LNL-AQP1 dTG (HS) mice after heat exposure. C: central vein of the hepatic lobules; P: portal triad.