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Immunostaining of 3-nitrotyrosine (3-NT) in the liver. A protein oxidative metabolite, 3-NT, was immunostained in the liver after 1 day of heat exposure. Minimal or no staining was observed in the 1st AB-free NC (inset of bottom) in the livers of Tie2-Cre/LNL-AQP1 dTG mice. Slight 3-NT-ir was detected in wild-type mice (top), and it was prominent in the vessels, including the sinusoids of the livers in Tie2-Cre/LNL-AQP1 dTG mice (bottom).
