Reagent	Final Concentration	Amount
Tris-HCL	30 mM	4.73 g
NaCl	30 mM	1.75 g
EDTA	0.1 mM	29.22 mg
H2O	n/a	1 L
Total	n/a	1L

^1.Dissolve Tris-HCL, NaCl, and EDTA in water while stirring.

^2.pH buffer to 8.0 at 37°C using 1M NaOH.