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| Reagent | Final Concentration | Amount |
|---|---|---|
| NaH2PO4 | 50 mM | 1.2 g |
| EDTA | 0.1 mM | 5.85 mg |
| Sodium Azide | 0.02% | 40 ug |
| H2O | 20% | 40 mL |
| 2H2O | 80% | 160 mL |
| Total | n/a | 200 mL |
Add NaH2PO4, EDTA, 0.02% sodium azide solution into 2H2O and H2O.
pH to 7.4 at 37°C using 1M NaO2H.
