Reagent	Final Concentration	Amount
2H2O	70%	700 mL
LB Broth	2%	20 g
Bacto Agar	1.5%	15 g
H2O	n/a	Fill to 1000 mL
Total	n/a	1000 mL

^1.Combine reagents in a flask and autoclave at 121°C, 15 PSI for at least 20 minutes.

^2.Allow the media to cool to ~55°C, then add 1000 uL of the kanamycin stock solution (1000x, 40 mg/ml)

^3.Pour ~25 mL of media per Petri plate (100 mm), repeat for remaining 1 L.