Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Reagent | Final Concentration | Amount |
|---|---|---|
| 2H2O | 70% | 35 mL |
| LB Broth | 2% | 1 g |
| H2O | n/a | Fill to 50 mL |
| Total | n/a | 50 mL |
Combine reagents in a flask and autoclave at 121°C, 15 PSI for at least 20 minutes.
Allow the media to cool to ~55°C, then add 50 uL of the kanamycin stock solution (1000x, 40 mg/ml)
