| Reagent | Final Concentration | Amount |
|---|---|---|
| NaH2PO4 | 50 mM | 6.9 g |
| KH2PO4 | 25 mM | 3.4 g |
| NaCl | 10 mM | 0.58 g |
| MgSO4 | 5 mM | 1.23 g |
| CaCl2 | 0.2 mM | 0.03 g |
| Bacterial Cell Media 10x | 0.1x | 10 mL |
| BME vitamins 100x | 0.25x | 2.5 mL |
| Studier trace metals 1000x | 0.25x | 0.25 mL |
| 15N-NH4CI | 1 g/L | 1 g |
| 2H-13C-glucose | 8 g/L | 8 g |
| BME vitamins | 0.25x | 2.5 mL |
| 2H2O | n/a | Fill to 50 mL |
| Total | n/a | 1000 mL |
1.
Combine reagents and add 1000 uL of the kanamycin stock solution (1000x, 90 mg/ml), and pH to 7.6 with NaO2H (Cambridge Isotope Laboratories)
2.
Sterile filter solution using a 1000 mL filtration system (Fisher Scientific)