Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Reagent | Final Concentration | Amount |
|---|---|---|
| IPTG | 0.5 M | 1.2 g |
| 2H2O | n/a | 10 mL |
| Total | n/a | 10 mL |
Completely dissolve IPTG in 2H2O
Sterilize solution using a 0.22 μm syringe filter (GenClone) and 10 mL syringe (BD)
Aliquot 1000 uL stocks and store at −20°C until use.
