| Reagent | Final Concentration | Amount |
|---|---|---|
| Tris-HCL | 30 mM | 4.73 g |
| NaCl | 30 mM | 1.75 g |
| H2O | n/a | 1 L |
| Total | n/a | 1L |
1.
Dissolve Tris-HCL and NaCl in water while stirring.
2.
pH buffer to 7.4 at 37°C using 1M NaOH.
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