Table 1.

Face-centered central composite design (FCCD) matrix representing silver nanoparticle biosynthesis by Fusarium oxysporum as affected by incubation time (X₁), initial pH level (X₂), AgNO₃ conc. (X₃), and temperature (X₄) with coded and actual factor levels.

Std	Run	Variables				The Yield (%)	AgNPs (µg/mL)		Residuals
		X1	X2	X3	X4		Experimental	Predicted
27	1	0	0	0	0	31.33	53.22	52.89	0.33
29	2	0	0	0	0	30.77	52.27	52.89	−0.62
5	3	−1	−1	1	−1	15.49	39.46	38.34	1.11
15	4	−1	1	1	1	28.9	73.65	72.91	0.74
10	5	1	−1	−1	1	50.67	43.04	41.71	1.33
9	6	−1	−1	−1	1	49.57	42.1	42.23	−0.13
11	7	−1	1	−1	1	48.86	41.5	41.54	−0.04
18	8	1	0	0	0	33.98	57.73	58.48	−0.75
16	9	1	1	1	1	38.19	97.32	96.72	0.6
28	10	0	0	0	0	31.64	53.74	52.89	0.85
21	11	0	0	−1	0	50.73	43.09	43.11	−0.02
1	12	−1	−1	−1	−1	64.52	54.8	55.5	−0.70
26	13	0	0	0	0	31.64	53.74	52.89	0.85
3	14	−1	1	−1	−1	43.16	36.66	36.29	0.37
6	15	1	−1	1	−1	23.36	59.51	59.57	−0.07
4	16	1	1	−1	−1	41.74	35.45	34.35	1.1
22	17	0	0	1	0	24.58	62.63	62.09	0.54
12	18	1	1	−1	1	47.62	40.45	41.59	−1.14
7	19	−1	1	1	−1	19.84	50.56	51.99	−1.43
14	20	1	−1	1	1	24.95	63.58	63.98	−0.40
2	21	1	−1	−1	−1	61.47	52.21	52.98	−0.77
20	22	0	1	0	0	32.99	56.04	56.34	−0.30
17	23	−1	0	0	0	28.91	49.11	47.83	1.28
19	24	0	−1	0	0	29.67	50.4	49.57	0.83
13	25	−1	−1	1	1	15.53	39.56	40.76	−1.20
24	26	0	0	0	1	32.6	55.38	55.13	0.25
25	27	0	0	0	0	30.77	52.27	52.89	−0.62
30	28	0	0	0	0	29.73	50.51	52.89	−2.37
23	29	0	0	0	−1	29.78	50.58	50.3	0.28
8	30	1	1	1	−1	29	73.9	73.8	0.1
Variable	Code	Coded and Actual Levels
		−1	0	1
Incubation time (h)	X1	24	48	72
Initial pH level	X2	5	6	7
AgNO3 conc. (mM/mL)	X3	0.5	1	1.5
Temperature (°C)	X4	30	35	40