Table 2.

Analysis of variance of CCD for silver nanoparticle biosynthesis by F. oxysporum as affected by incubation time (X₁), initial pH level (X₂), AgNO₃ conc. (X₃), and temperature (X₄).

Source of Variance		Coefficient Estimate	Sum of Squares	Degrees of Freedom	Mean Square	F-Value	p-Value
Model	Intercept	52.89	4678.97	14	334.21	220.94	<0.0001 *
Linear effect	X1	5.32	509.81	1	509.81	337.02	<0.0001 *
	X2	3.38	205.85	1	205.85	136.08	<0.0001 *
	X3	9.49	1621.85	1	1621.85	1072.17	<0.0001 *
	X4	2.41	104.85	1	104.85	69.31	<0.0001 *
Interaction effect	X1 X2	0.14	0.34	1	0.34	0.22	0.6442
	X1 X3	5.94	563.90	1	563.90	372.78	<0.0001 *
	X1 X4	0.50	3.98	1	3.98	2.63	0.1258
	X2 X3	8.21	1079.34	1	1079.34	713.52	<0.0001 *
	X2 X4	4.63	342.69	1	342.69	226.54	<0.0001 *
	X3 X4	3.92	245.91	1	245.91	162.56	<0.0001 *
Quadratic effect	X12	0.27	0.19	1	0.19	0.12	0.7301
	X22	0.07	0.01	1	0.01	0.01	0.9281
	X32	−0.29	0.21	1	0.21	0.14	0.7117
	X42	−0.17	0.08	1	0.08	0.05	0.8258
Error effect	Lack of Fit		15.15	10	1.51	1.00	0.5327
	Pure Error		7.54	5	1.51
R2	0.9952
Adj R2	0.9907
Pred R2	0.9745
Adeq Precision	71.71

* Significant values, F: Fishers’s function, p: level of significance.