TABLE 1.
Cells | Development of CPEs inb
|
|||||
---|---|---|---|---|---|---|
MV strains
|
CDV strains
|
RPV strain Ako | ||||
Edmonston | KA | Onderstepoort | HA7 | 851 | ||
CHO | − | − | − | − | − | ND |
Vero | ++ | − | + | − | − | −c |
B95a | ++ | ++ | − | + | + | ND |
CHO.Neo | − | − | − | − | − | −c |
CHO.SLAM | ++ | ++ | − | ++ | ++ | ++ |
CHO.DogSLAMtag | ++ | + | ++ | ++ | ++ | ++ |
CHO.CowSLAMtag | ++ | ++ | ++ | + | ++ | +++ |
Cell lines were infected with each morbillivirus strain at an MOI of 0.1, and development of CPEs was assessed under a microscope at 24 h after infection with MV and CDV strains and at 12 h after infection with RPV. The susceptibilities of CHO, Vero, and B95a cells to MV strains have been described elsewhere (43). Longer incubation did not affect the presence or absence of CPEs in cells except those infected with the Ako strain.
−, no syncytia found; +, syncytia found in some of the fields; ++, syncytia found in most fields; +++, the majority of cells detached from the plate because of extensive cell fusion; ND, not done.
Cells developed CPEs after more than 15 h of incubation.