Extended Data Fig. 4. Aster-A undergoes post-translational stabilization in ECs after activation.

(a) GRAMD1A (Aster-A), GRAMD1B (Aster-B) and GRAMD1C (Aster-C) expression relative to 36B4 in primary HAECs. (b) qPCR for SELE, VCAM1, ICAM1 and GRAMD1A (Aster-A) in immortalized HAECs treated with TNFα for 0–24 h. (c) Endogenous Aster-A and ICAM-1 protein levels in HAECs treated with TNFα (10 ng/ml) for 0–24 h. (d) Western blots for His-ALOD4 and HA-Aster-A in HAECs stably overexpressing HA-Aster-A or GFP after LPS exposure for 0–24 h. Data are represented as mean ± SEM.