Extended Data Fig. 6. HPCD infusions lower VCAM-1 in response to LPS in vivo.

(a) Western blots for VCAM-1 in the lungs of male F/F and ECKO mice 3 weeks after Cre induction. (b) Total plasma cholesterol in male F/F and ECKO mice injected with LPS (60 μg/mouse) for 20 mins before receiving i.v infusions of saline or HPCD (60 mg/mouse). Blood and tissues were collected 3 h after LPS injections. n = 5 F/F + saline, 5 ECKO + saline, 6 F/F + HPCD and 6 ECKO + saline. (c) qPCR in the hearts of male F/F and ECKO mice injected with LPS (60 μg/mouse) for 20 mins before receiving i.v infusions of saline or HPCD (60 mg/mouse). Tissues were collected 3 h after LPS injections. n = 5 F/F + saline, 5 ECKO + saline, 6 F/F + HPCD and 6 ECKO + saline. Data are represented as mean ± SEM with individual mice noted as dots.