Figure 2. Removal of the ER C-terminal tail domain leads to hyperactivity in promoting pedicel elongation and inhibiting stomatal development.

(A) Representative pedicels and mature siliques of WT, er-105 (er), ER-FLAG er, ER_ΔCTα_-Helix-FLAG er, and ER_ΔC43-FLAG er plants. For each transgenic constructs, two representative lines were subjected to analysis. Images were taken under the same magnification. Scale bar, 1 cm.

(B) Morphometric analysis of pedicel length from each genotype. Six-week-old mature pedicels (n = 10) were measured. One-way ANOVA followed by Tukey’s HSD test was performed and classified their phenotypes into categories (e.g. a, b, c).

(C) Representative confocal microscopy of cotyledon abaxial epidermal at 7 day-post-germination (DPG7) from wild type (WT), er-105 (er), ER-FLAG er, ER_ΔCTα-Helix-FLAG er, and ER_ΔC43-FLAG er. The same representative transgenic lines were observed. Images were taken under the same magnification. Scale bar = 50 μm.

(D) Quantitative analysis. Stomata + meristemoid index (number of stomata and meristemoid per 100 epidermal cells) of the cotyledon abaxial epidermis from 7-day-old seedlings of respective genotypes (n = 10). One-way ANOVA followed by Tukey’s HSD test was performed and classified their phenotypes into categories (e.g. a, b, c).