Table 10.
Overview of in vitro studies related to resveratrol.
| Polyphenol | Cell Line | Concentration/ Duration |
Anti-Cancer Effects | Type IV Collagenase-Mediated Mechanisms | Reference |
|---|---|---|---|---|---|
| Resveratrol (3,5,4′-trihydroxystilbene) |
PMA-induced A549 HeLa |
10, 30 µM for 24 h | ↓proliferation ↓cell migration ↓cell invasion |
↔MMP-2 mRNA, activity ↓MMP-9 mRNA, activity ↓NF-κB activity |
[116] |
| 4 T1 | 10, 20, 30 μM for 12, 16, 24, 48 h | ↓cell adhesion ↓cell migration ↓cell invasion |
↓MMP-9 mRNA, activity | [135] | |
| HT1080 | 50 μM for 10, 30 min, 1, 3, 6, 12, 24 h or 10, 20, 30, 40, 50 μM for 24 h or 20, 30, 50 μM for 24, 48 h |
↓proliferation ↑cell migration |
↑MMP-9 protein, activity ↓pp38 ↓pAkt |
[134] | |
| BxPC-3 Panc-1 |
12.5, 25, 50 μM for 24, 48 h | ↓proliferation ↓cell migration ↓cell invasion |
↓MMP-2, -9 mRNA, protein ↓pNF-κB protein ↓pAkt protein |
[123] | |
| HTB94 | 50 μM for 10, 30 min, 1, 3, 6, 12, 24 h or 10, 20, 30, 40, 50 μM for 24 h |
↓proliferation | ↓MMP-2, -9 protein, activity ↑pp38 |
[133] | |
| TGF-β1-induced LoVo | 6, 12 μM for 24, 48 h | ↓proliferation ↓cell migration ↓cell invasion |
↓MMP-2, -9 protein | [139] | |
| Glioblastoma-initiating cells (GICs) derived from patients | 5, 10, 20 μM for 24, 48 h | ↓proliferation ↓cell adhesion ↓cell migration ↓cell invasion |
↓MMP-2 protein, activity ↓NF-κB p65 nuclear translocation ↑I-κBα, ↓pI-κBα protein ↑IKKα/β, ↓pIKKα/β protein ↓pAkt, ↓pmTOR protein |
[141] | |
| TPA-induced SCC-9 |
25, 50, 75, 100 μM for 24 h | ↓proliferation ↓cell migration ↓cell invasion |
↔MMP-2 activity ↓MMP-9 mRNA, protein, activity ↓pERK protein |
[113] | |
| C6 | 50, 100, 150 μM for 24, 48 h | ↓proliferation ↑apoptosis |
↓MMP-9 protein ↓PI3 K, pAkt, mTOR, STAT3 protein ↓NF-κB protein |
[140] | |
| HOS MNNG/HOS 143 B |
25, 50, 75, 100 μM for 12, 24, 48 h or 100 μM for 2, 4 h |
↓proliferation ↓cell adhesion ↓cell migration ↓cell invasion |
↓MMP-2 mRNA, protein, activity, promoter activity ↔TIMP-2 protein ↓pp38, pAkt protein ↑pERK protein |
[142] | |
| SW579 | 10 μg/mL for 24 h with 100, 200 μg/mL of lentinan |
↓proliferation ↑apoptosis |
↓MMP-2, -9 mRNA ↑TIMP-1, -2 mRNA ↓NF-κB mRNA ↑I-κBα mRNA |
[131] | |
| Hypoxia-induced BxPC-3 Panc-1 |
12.5, 25, 50 μM for 24, 48 h | ↓proliferation ↓cell migration ↓cell invasion |
↓MMP-2 mRNA, protein | [124] | |
| TPA-stimulated MCF-7 | 10 μM for 1, 24 h (resveratrol) 1, 5, 10 μM for 1, 4, 12, 24 h (gold-conjugated resveratrol nanoparticles) |
↓proliferation ↓cell migration ↓cell invasion |
↓MMP-2 protein ↓MMP-9 mRNA, protein, activity, secretion ↑TIMP-1, -2 protein ↓NF-κB p65/pNF-κB p65 protein ↓pAkt, pERK protein |
[117] | |
| U87 MG T98 G U251 |
40 μM for 24, 48 h | ↓proliferation ↓cell migration ↓cell invasion |
↓MMP-2 protein, activity | [112] | |
| T24 | 10, 25, 50 μM for 6, 12, 24 h | ↓cell migration ↓cell invasion |
↓MMP-2, -9 protein ↓pERK-1/-2 protein |
[129] | |
| SW480 HCT116 |
5 μM for 12, 24 h or 10, 28 days | ↓proliferation ↓cell invasion (in alginate culture) ↑apoptosis |
↓MMP-9 protein ↓pNF-κB p65 protein ↓pNF-κB p50 protein |
[121] | |
| HepG2 | 10 μg/mL for 24 h with 5, 10 μg/mL of paclitaxel |
↑apoptosis | ↓MMP-2, -9 mRNA, protein ↑TIMP-1, -2 mRNA, protein ↓NF-κB mRNA, protein ↑I-κBα mRNA, protein |
[125] | |
| U2 OS | 6, 12 μg/mL for 24, 48 h | ↓proliferation ↓cell migration ↓cell invasion ↑apoptosis |
↓MMP-2, -9 protein | [132] | |
| HCT116 5-Fluorouracil-resistant HCT116 R |
5 μM for 24, 72 h or 10 days | ↓proliferation ↓cell invasion (in alginate culture) ↑apoptosis |
↓MMP-9 protein ↓pNF-κB p65 protein |
[122] | |
| Cancer-associated fibroblasts (CAF)-induced MCF-7 MDA-MB-231 |
50 μM for 24, 36, 48 h | ↓proliferation ↓cell migration ↓cell invasion |
↓MMP-2, -9 mRNA ↓pAkt |
[119] | |
| IL-6-induced SGC-7901 HSC-39 |
10, 20 μM for 48 h | ↓proliferation ↓cell invasion |
↓MMP-2, -9 activity ↓pERK protein |
[138] | |
| ACHN A498 |
25, 50, 100 μM for 24, 48 h | ↓proliferation ↓cell migration ↓cell invasion |
↓MMP-2, -9 protein ↑TIMP-1 protein ↓pAkt, ERK-1/-2, pERK-1/-2 |
[127] | |
| TGF-1 β-induced MDA-MB-231 MDA-MB-436 MDA-MB-453 BT549 |
12.5, 25, 50 μM for 24 h | ↓proliferation ↓cell migration ↓cell invasion |
↓MMP-2 protein, secretion (MDA-MB-231) ↓MMP-9 protein, secretion (MDA-MB-231) ↓pAkt, pPI3 K |
[118] | |
| HeLa Cancer Stem Cells (CSCs) |
10, 20, 40 μM for 24, 48 h | ↓proliferation ↑cell migration (slightly, after 24 h) ↓cell migration (after 48 h) ↓cell invasion ↑apoptosis |
↓MMP-2, -9 protein | [120] | |
| PC3 DU145 LNCap |
150 μM for 12, 24, 48 h | ↓cell migration ↓cell invasion |
↓MMP-2, -9 protein ↑TIMP-2, -3 protein ↓TIMP-2, -3 methylation level |
[130] | |
| Cisplatin-resistant CAL27 | 25, 50, 75 μM for 6, 12, 18, 24 h | ↓proliferation ↓cell migration ↓cell invasion |
↓MMP-2, -9 protein ↓pERK, pp38 protein |
[114] | |
| H-357 | 1, 3.5, 5 μM for 48 h (resveratrol nanoparticles) | ↓angiogenesis ↓proliferation ↓cell invasion |
↓MMP-2, -9 activity ↓NF-κB, IKKα protein ↓Akt, MAPK protein |
[137] | |
| Paclitaxel-resistant SKBR3/PR |
30 μM for 24, 48 h (free resveratrol) or equivalent dose (resveratrol–solid lipid nanoparticles with or without TPGS) | ↓proliferation ↓cell migration ↓cell invasion ↑apoptosis |
↓MMP-2, -9 protein | [136] |
Legend: ↑ activation or increase; ↓ inhibition or decrease; ↔ no impact.