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Transcomplementation assay strategy. (A) EREB2.5 cells grown in the absence of estrogen result in EREBNA2 molecules becoming inactivated. As a result, the cells are unable to survive. (B) EREB2.5 cells transduced with pLIG.EBNA2 virus express wild-type EBNA2. Withdrawal of estrogen results in inactivation of the EREBNA2 proteins; however, the cells survive since wild-type EBNA2 is provided in trans.
