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. 2001 Jul;75(13):5899–5912. doi: 10.1128/JVI.75.13.5899-5912.2001

FIG. 8.

FIG. 8

Detection of EBNAs, LMP-1, and c-Myc in EREB2.5 cells expressing high levels of Notch1IC after estrogen withdrawal. (A) Western blot of cellular extracts derived from cells starved for estrogen for 4 days. The blot was probed with the PE2 monoclonal antibody directed against EBNA2. Both EREBNA2 and wild-type EBNA2 genes can be detected and are indicated by arrows. The cell lines used are indicated above the blot. Lane 5, EREB2.5 cells grown in media containing estrogen; lane 6, DG75 cells. (B) Same as for panel A, except that the blot was probed with an anti-Notch1 antisera. (C) Same as for panel A, except that the blot was probed with an anti-EBNA-1 antibody. (D) Same as for panel A, except that the blot was probed with an anti-EBNA-3A antibody. (E) Same as for panel A, except that the blot was probed with an anti-c-myc antibody. (F) Same as for panel A, except that the blot was probed with an anti-LMP-1 antibody.