FIG. 1.

(A) Cytopathic effects in infected cells. The mutant virus H5ts116 was utilized to infect various G-418-resistant cell lines at the permissive temperature of 32°C or the nonpermissive temperature of 39°C. The K-16 cell line supported growth of the virus, as evidenced by the onset of cytopathic effect (+++) at both temperatures. 0 = no cytopathic effect. (B) Detection of 100K-specific DNA sequences within K-16 cells and not 293 cells. A 100K-specific PCR product at ∼2.3 kb was only detected when DNA isolated from the G-418-resistant K-16 cells was utilized as a template. The first lane contains a 1-kb DNA ladder, while the control lane utilized pcDNA3/100K as a positive control template. See Materials and Methods for full experimental details. (C) Detection of 100K-specific RNA sequences within K-16 cells. A 100K-specific mRNA was detected only in RNA isolated from G-418-resistant K-16 cells. The lower half of the figure depicts the amounts of RNA loaded in the gel prior to transfer to the nylon membrane, demonstrating that both samples contained equal amounts of intact RNA. See Materials and Methods for full experimental details.