Figure 1.

Construction of the Rice Chromosome 4 Tiling DNA Microarray.

(A) A total of 14,742 PCR-amplified overlapping fragments, which were selected to cover the entire chromosome, were printed onto glass slides with negative and positive controls. An image of four subarrays of a sample microarray hybridized with probes originating from seedling shoots labeled with Cy3 and cultured cells labeled with Cy5 is presented. The bottom row of each subarray contains negative control spots.

(B) Quality-control gel image of 96 PCR-amplified fragments from one randomly chosen 96-well plate.