Figure 3.

RNA Gel Blot Analysis Shows That SE-WAP41 mRNA Levels Decrease When Etiolated Maize Seedlings Are Exposed to Light.

(A) Five-day-old etiolated maize seedlings were exposed to white light for 3, 6, and 24 h or kept in the dark for 24 h. A blot of total RNA extracted from the upper 1 cm of mesocotyls was hybridized to cDNAs of SE-WAP41, actin, and 26S-rRNA. The ribosomal 26S rRNA probe served as a loading control. The decreased actin mRNA levels is indicative of cell division cessation.

(B) Relative SE-WAP41 mRNA levels. Values were calculated relative to 26S-rRNA intensity in each lane.