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. 2005 Jun;17(6):1801–1814. doi: 10.1105/tpc.105.031419

Figure 5.

Figure 5.

CmPP16-1 Interacts with Specific Pumpkin Phloem Sap Proteins.

(A) and (B) Comparison of gel-filtration profiles of QCmPP (top gel) and Pn16 (bottom gel) (A) and Pn16 (top gel) and Remix (bottom gel) (B). The inset in (B) shows protein profiles of Pn16 (lane 1) and a fraction without CmPP16 proteins (lane 2). Protein samples were run on a 12.5% acrylamide gel. In each gel, the top images show protein stain by Coomassie Brilliant Blue (PS) and the bottom images show immunoblotting using anti-CmPP16-1 antibody (IB). Arrows indicate CmPP16-1. Gel-filtration elution fractions were grouped into FM (10 to 25 kD), FD (25 to 40 kD), and FH (>40 kD). In QCmPP, CmPP16-1 was eluted in FM, FD, and FH. In Pn16, most of the CmPP16-1 was eluted in FM.

(C) and (D) Immunoprecipitation assay using anti-CmPP16-1 antibody against QCmPP-derived FH, FD, and FM (C) and Remix fraction (D). Input proteins were immunoprecipitated using either anti-CmPP16-1 IgG (Anti-16-1) or preimmune IgG (Pre). Protein samples were run on a 12.5% acrylamide gel and silver-stained. The numbered bands represent coimmunoprecipitated proteins. Asterisks and double asterisks indicate CmPP16-1 monomer and CmPP16-1 dimer, respectively.