Figure 8.

The Presence of Interacting Proteins Did Not Positively Regulate the Root-Ward Movement of CmPP16-2.

(A) Effect of FD addition on CmPP16-2 movement. Phloem-purified CmPP16-2 was mixed with native FD and biotinylated to obtain B-FD2. B-FD2 tracer (Tr) was introduced, and then B-HisGFP (green ovals), B-CmPP16-1 (blue ovals), and B-CmPP16-2 (yellow ovals) movement was estimated by 2DE-ABC in distant leaves (DL) and roots (R).

(B) Comparison of the relative signal intensity of B-CmPP16-2 (yellow bars) and B-CmPP16-1 (blue bars). Values represent means ± se from three independent plants. Values of B-Pn16 introduction were adapted from Figure 4C. In B-FD2 introduction, the relative signal intensity of B-CmPP16-2 in roots was much smaller than that in B-Pn16 and similar to that of B-FD2 tracer, indicating that the root-ward movement of B-CmPP16-2 was not positively regulated in the presence of FD proteins. B-CmPP16-1 was clearly detected in roots, indicating that the root-ward movement was restored, as seen in Figure 6B.