Figure 3.

p90RSK is able to regulate rate of proliferation in A375 and A549 cell lines. (A) Growth curves of A375 and A549 cells seeded in culture medium with variable concentrations of BI-D1870. The experiments were repeated three times. The curves show the averages of three different measurements ± standard deviation. (B) A375, A549 and LNCaP cells, treated or not with 4 µM BI-D1870 or with 2 µM wortmannin for 72 h, were photographed in the optical field at 10× magnification. A representative area is shown. (C) Growth curves of A549 and A375 cells transfected with a combination of different RSK1/RSK2 specific siRNA or with non-targeting siRNA (siCTR). The efficiency of transfection was measured with anti-RSK1 and anti-RSK2 antibodies. Normalization was performed with an antibody against tubulin. The experiments were conducted three different times. The histograms show the average value of the obtained results at 0 and 48 h and the standard deviation.