Table 1.
Key resources table.
| REAGENT | SOURCE | IDENTIFIER | COMMENT |
|---|---|---|---|
| Antibodies | |||
| S9.6 | Kerafast (USA) | ENH002 | Lot 202301 |
| S9.6 | ActiveMotif (Belgium) | 65683 | Lot 35021002 |
| Goat anti-mouse Alexa Fluor 647 | Thermo Fisher Scientific (USA) | A-21236 | |
| Mouse anti-BrdU antibody | BD Biosciences (USA) | 347580 | Stains IdU |
| Rat anti-BrdU antibody | Abcam (UK) | Ab6326 | Stains CldU |
| Anti-mouse Cy3 | Rockland (USA) | 610-404-040 | |
| Anti-rat BV480 | BD Biosciences | 564878 | |
| Mouse anti-ssDNA | Developmental Studies Hybridoma Bank (USA) | Anti-ssDNA | Lot 5/11/17–37 ug/mL |
| Anti-mouse Alexa Fluor 488 | Thermo Fisher Scientific | A-11001 | |
| Chemicals and other reagents | |||
| CldU | Sigma (USA) | C6891-100MG | Lot MKCJ7518 |
| IdU | Sigma | I7125-25G | Lot BCBP3111V |
| BSA | Sigma | A9647 | |
| BlockAid | Thermo Fisher Scientific | B10710 | |
| Triton-X100 | Sigma | 9002-93-1 | |
| Tween-20 | Sigma | P1379 | |
| RNAse A | Thermo Fisher Scientific | 12091021 | |
| RNAse H | New England Biolabs (USA) | M0297L | |
| 4% Paraformaldehyde solution in PBS | Thermo Fisher Scientific | J19943.K2 | |
| NaOH | Thermo Fisher Scientific | S318 | |
| Ethyl alcohol | Thermo Fisher Scientific | BP28184 | |
| ProlongGold | Thermo Fisher Scientific | P10144 | |