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. 2024 Aug 27;14(9):416. doi: 10.3390/bios14090416

Figure 1.

Figure 1

(a) ELISA assay results curves for detection of recombinant N protein with 2 and 0.2 µg/mL horse anti-N pAbs showing the recognition of the N protein. (b) Half-strip assays testing sample buffer with (Spiked, S) and without (Non-spiked, NS) 10 ng N protein addition, showing the performance of 2 µg/strip of horse anti-N pAbs as the capture element in the test line (TL) and Protein A in the control line (CL) before and after N protein column affinity purification with AuNPs-horse anti-N conjugate. (c) ELISA assay results curves for detection of recombinant N protein with 1.5 and 0.15 µg/mL llama anti-N pAbs showing the recognition of the N protein. (d) Half-strip assays testing sample buffer with (Spiked, S) and without (Non-spiked, NS) 10 ng N protein addition showing the performance of 1.5 µg/strip llama anti-N Abs as the capture element in the test line (TL) and Protein A in the control line (CL) before and after N protein column affinity purification with AuNPs-horse anti-N conjugate. (e) ELISA assay results curves for detection of recombinant N protein with 2.1 and 0.21 µg/mL mouse anti-N mAbs showing the recognition of the N protein. (f) Half-strip assays testing sample buffer with (Spiked, S) and without (Non-spiked, NS) 10 ng N protein addition showing the performance of 2.1 µg/strip mouse anti-N mAbs as the capture element in the test line (TL) and Protein A in the control line (CL) before and after Protein G column affinity purification with AuNPs-horse anti-N conjugate (left) compared to the same Ab as the detection element in the AuNPs-Ab conjugate using strips with 1 µg/strip horse anti-N Ab as the capture element (right).