FIG. 3.

Intracellular expression of HSV-1 proteins by immature MDDC. Immature MDDC were infected with HSV-1 at 5 PFU/cell and analyzed for expression of immediate-early (ICP4), early (ICP8), and late (gB, gC, and gD) HSV-1 antigens at selected times. (A) ICP4 at 4 h p.i., showing cytoplasmic staining; (B) cytoplasmic staining with ICP8 at 6 h p.i.; (C) gD at 10 h p.i., showing cytoplasmic staining; and gB at 12 h p.i. (D) and gC staining at 18 h p.i.(E), showing cell surface staining. Mock-infected cells did not stain for any of the tested antibodies (not shown). Bar, 10 μm.